My research interests

Protein : RNA recognition

Integrative structural biology space

RNAs constitute a rich family of biolomecules that include for instance ribosomal, transfer, small regulatory, viral genomic RNAs or ribozymes (RNA catalysts). They are involved in processes such as the regulation of gene expression, the translation of genetic information into proteins, the maturation of other RNAs. I am particularly interested in transfer RNAs (tRNAs), which act as molecular adapters, during protein synthesis. I study their maturation, stability, and the way they interact with proteins through a multidisciplinary approach based on biochemical and biophysical methods. Indeed, beside X-ray crystallography, analyses made in solution - i.e. size exclusion chromatography (SEC), dynamic light scattering (DLS), circular dichroism (SRCD), small angle X-ray scattering (SAXS) - provide very complementary structural data to understand the properties and the multiple functions of such ribonucleoproteic systems. Among others, my work has led to the structural characterization of :


Crystals vide Crystallography is my favorite investigation tool to study the 3D structure of biomolecules, but it requires the production of "good" crystals. This is why I take an interest in this field of biological crystallogenesis, the science (the art?) of crystal growth.

Over the last twenty years, I have been involved in the development of methods that facilitate the preparation of crystals for crystallographic studies like:

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